GrapeTree is an integral part of EnteroBase and we advise that you use GrapeTree through EnteroBase for the best results. However, many people have asked for a stand-alone GrapeTree version that they could use offline or integrate into the other applications.
The stand-alone version emulates the EnteroBase version through a lightweight webserver running on your local computer. You will be interacting with the program as you would in EnteroBase; through a web browser. We recommend Google Chrome for best results.
For detailed help please see: http://enterobase.readthedocs.io/en/latest/grapetree/grapetree-about.html
For a formal description, please see the preprint: https://www.biorxiv.org/content/early/2017/11/09/216788
There are number of different ways to interact with GrapeTree, for best results install via pip :
pip install grapetree grapetree
We also have ready-made binaries for download here: https://github.com/achtman-lab/GrapeTree/releases
Running on Mac: Download GrapeTree_mac.zip
You will need to unzip GrapeTree_mac.zip (just double click). Inside there will be an app you can drag into your Applications folder. You may be warned about Security settings, if you right click on the GrapeTree app and then click “Open” it should be fine.
Running on Windows: Download GrapeTree_win.zip
Once downloaded, you will need to untzip GrapeTree_win.zip and then open the extracted folder and run GrapeTree_win.exe. When you run it the first time on windows you might get a prompt about security. On Windows 10, click the small text: “More info”, and then the button “Run Anyway”.
Running from Source code
GrapeTree requires Python 2.7 and some additional python modules (listed in requirements.txt). The easiest way to install these modules is with pip:
pip install -r requirements.txt chmod +x binaries/
On Linux or MacOSX you need to make sure the binaries in binaries/ can be executed. To run GrapeTree;
\GrapeTree>python main.py * Running on http://127.0.0.1:8000/ (Press CTRL+C to quit)
The program will automatically open your web browser and you will see the GrapeTree Splash Screen. If at anytime you want to restart the page you can visit http://localhost:8000 in your web browser. To view a tree (newick or Nexus) or create a tree from an allele profile, just drag and drop the file into the browser window.
Runtime behaviour can be configured in grapetree/config.py.
To run tests, run pytests in the top level directory.
usage: grapetree [-h] --profile PROFILE [--method METHOD] [--matrix MATRIX_TYPE] [--recraft] [--missing HANDLE_MISSING] [--wgMLST] [--heuristic HEURISTIC] [--n_proc N_PROC] [--check] Parameters for command line version of GrapeTree. You can drag the Newick output into the web interface. optional arguments: -h, --help show this help message and exit --profile PROFILE, -p PROFILE A file contains either MLST / SNP profiles or multile aligned sequences in fasta format. --method METHOD, -m METHOD backend algorithms to call. Allowed values are "MSTreeV2" [default]: Direct minimum spanning tree with post-correction, "MSTree": Standard minimum spanning tree, "NJ": Standard NJ implemented in FastME V2, "distance": distance matrix in PHYLIP format --matrix MATRIX_TYPE, -x MATRIX_TYPE Either "symmetric" [default for MSTree and NJ] or "asymmetric" [default for MSTreeV2]. --recraft, -r Allows local branch recrafting after tree construction. Default in MSTreeV2. --missing HANDLE_MISSING, -y HANDLE_MISSING Alternative ways of handling missing data. 0: missing data are ignored in pairwise comparisons [default]. 1: Columns that have missing data are ignored in the whole analysis. 2: missing data are treated as a special value (allele). 3: Naive counting of absolute differences between profiles. --wgMLST, -w Experimental option for a better support of wgMLST schemes. --heuristic HEURISTIC, -t HEURISTIC Tiebreak rules between co-optimal edges. Only used in MSTree [default: eBurst] and MSTreeV2 [default: harmonic] --n_proc N_PROC, -n N_PROC Number of processes. Default: 5. --check, -c Do not calculate the tree but only show the expected time/memory consumption.
python grapetree.py -p examples/simulated_data.profile -m MSTreeV2
python grapetree.py -p examples/simulated_data.profile -m NJ
python grapetree.py -p examples/simulated_data.profile -m distance
Copyright Warwick University This program is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version.
This program is distributed in the hope that it will be useful, but without any warranty; without even the implied warranty of merchantability or fitness for a particular purpose. See the GNU General Public License for more details.
You should have received a copy of the GNU General Public License along with this program. If not, see http://www.gnu.org/licenses/.
Detailed information for the standard NJ implemented in FastME V2: http://www.atgc-montpellier.fr/fastme/
EnteroMSTree - GrapeTree has not been formally published yet. If you use GrapeTree please cite the preprint:
Z Zhou, NF Alikhan, MJ Sergeant, N Luhmann, C Vaz, AP Francisco, JA Carrico, M Achtman (2017) “GrapeTree: Visualization of core genomic relationships among 100,000 bacterial pathogens”, bioRxiv 216788; doi: https://doi.org/10.1101/216788